In Vivo Molecular Optical Coherence Tomography of Lymphatic Vessel Endothelial Hyaluronan Receptors
Identifieur interne : 000872 ( Main/Exploration ); précédent : 000871; suivant : 000873In Vivo Molecular Optical Coherence Tomography of Lymphatic Vessel Endothelial Hyaluronan Receptors
Auteurs : Peng Si [États-Unis] ; Debasish Sen [États-Unis] ; Rebecca Dutta [États-Unis] ; Siavash Yousefi [États-Unis] ; Roopa Dalal [États-Unis] ; Yonatan Winetraub [États-Unis] ; Orly Liba [États-Unis] ; Adam De La Zerda [États-Unis]Source :
- Scientific Reports [ 2045-2322 ] ; 2017.
Abstract
Optical Coherence Tomography (OCT) imaging of living subjects offers increased depth of penetration while maintaining high spatial resolution when compared to other optical microscopy techniques. However, since most protein biomarkers do not exhibit inherent contrast detectable by OCT, exogenous contrast agents must be employed for imaging specific cellular biomarkers of interest. While a number of OCT contrast agents have been previously studied, demonstrations of molecular targeting with such agents in live animals have been historically challenging and notably limited in success. Here we demonstrate for the first time that microbeads (µBs) can be used as contrast agents to target cellular biomarkers in lymphatic vessels and can be detected by OCT using a phase variance algorithm. This molecular OCT method enables
Url:
DOI: 10.1038/s41598-017-01172-x
PubMed: 28439123
PubMed Central: 5430649
Affiliations:
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xml:lang="en"><p>Optical Coherence Tomography (OCT) imaging of living subjects offers increased depth of penetration while maintaining high spatial resolution when compared to other optical microscopy techniques. However, since most protein biomarkers do not exhibit inherent contrast detectable by OCT, exogenous contrast agents must be employed for imaging specific cellular biomarkers of interest. While a number of OCT contrast agents have been previously studied, demonstrations of molecular targeting with such agents in live animals have been historically challenging and notably limited in success. Here we demonstrate for the first time that microbeads (µBs) can be used as contrast agents to target cellular biomarkers in lymphatic vessels and can be detected by OCT using a phase variance algorithm. This molecular OCT method enables <italic>in vivo</italic> imaging of the expression profiles of lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1), a biomarker that plays crucial roles in inflammation and tumor metastasis. <italic>In vivo</italic> OCT imaging of LVYE-1 showed that the biomarker was significantly down-regulated during inflammation induced by acute contact hypersensitivity (CHS). Our work demonstrated a powerful molecular imaging tool that can be used for high resolution studies of lymphatic function and dynamics in models of inflammation, tumor development, and other lymphatic diseases.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="James, Ml" uniqKey="James M">ML James</name></author><author><name sortKey="Gambhir, Ss" uniqKey="Gambhir S">SS Gambhir</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, D" uniqKey="Huang D">D Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, J" uniqKey="Kim J">J Kim</name></author><author><name sortKey="Brown, W" uniqKey="Brown W">W Brown</name></author><author><name sortKey="Maher, Jr" uniqKey="Maher J">JR Maher</name></author><author><name sortKey="Levinson, H" uniqKey="Levinson H">H Levinson</name></author><author><name sortKey="Wax, A" uniqKey="Wax A">A 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sortKey="De La Zerda, Adam" sort="De La Zerda, Adam" uniqKey="De La Zerda A" first="Adam" last="De La Zerda">Adam De La Zerda</name><name sortKey="De La Zerda, Adam" sort="De La Zerda, Adam" uniqKey="De La Zerda A" first="Adam" last="De La Zerda">Adam De La Zerda</name><name sortKey="De La Zerda, Adam" sort="De La Zerda, Adam" uniqKey="De La Zerda A" first="Adam" last="De La Zerda">Adam De La Zerda</name><name sortKey="De La Zerda, Adam" sort="De La Zerda, Adam" uniqKey="De La Zerda A" first="Adam" last="De La Zerda">Adam De La Zerda</name><name sortKey="Dutta, Rebecca" sort="Dutta, Rebecca" uniqKey="Dutta R" first="Rebecca" last="Dutta">Rebecca Dutta</name><name sortKey="Dutta, Rebecca" sort="Dutta, Rebecca" uniqKey="Dutta R" first="Rebecca" last="Dutta">Rebecca Dutta</name><name sortKey="Liba, Orly" sort="Liba, Orly" uniqKey="Liba O" first="Orly" last="Liba">Orly Liba</name><name sortKey="Liba, Orly" sort="Liba, Orly" uniqKey="Liba O" first="Orly" last="Liba">Orly Liba</name><name sortKey="Liba, Orly" sort="Liba, Orly" uniqKey="Liba O" first="Orly" last="Liba">Orly Liba</name><name sortKey="Liba, Orly" sort="Liba, Orly" uniqKey="Liba O" first="Orly" last="Liba">Orly Liba</name><name sortKey="Sen, Debasish" sort="Sen, Debasish" uniqKey="Sen D" first="Debasish" last="Sen">Debasish Sen</name><name sortKey="Sen, Debasish" sort="Sen, Debasish" uniqKey="Sen D" first="Debasish" last="Sen">Debasish Sen</name><name sortKey="Si, Peng" sort="Si, Peng" uniqKey="Si P" first="Peng" last="Si">Peng Si</name><name sortKey="Winetraub, Yonatan" sort="Winetraub, Yonatan" uniqKey="Winetraub Y" first="Yonatan" last="Winetraub">Yonatan Winetraub</name><name sortKey="Winetraub, Yonatan" sort="Winetraub, Yonatan" uniqKey="Winetraub Y" first="Yonatan" last="Winetraub">Yonatan Winetraub</name><name sortKey="Winetraub, Yonatan" sort="Winetraub, Yonatan" uniqKey="Winetraub Y" first="Yonatan" last="Winetraub">Yonatan Winetraub</name><name sortKey="Yousefi, Siavash" sort="Yousefi, Siavash" uniqKey="Yousefi S" first="Siavash" last="Yousefi">Siavash Yousefi</name><name sortKey="Yousefi, Siavash" sort="Yousefi, Siavash" uniqKey="Yousefi S" first="Siavash" last="Yousefi">Siavash Yousefi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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